Important !!!! Courses are for the current Students of JWU who are pursuing UG or PG classes in any semester and any stream( Arts/ Science/ Commerce)

Eligibility Criteria for Admission in PLANT TISSUE CULTURE TECHNOLOGY
CERTIFICATE COURSE ON Plant tissue culture technology
(1 year PROGRAMME)
Information Dates
1. Eligibility for Admission Bachelor’s Degree with any stream,
Preference Given to B.Sc. Biotechnology,
, B.Sc. Botany and B.Sc. Agriculture with
45% Marks
2. Fee structure Rs 15,000 /- Per semester
3. Course Duration 2 Semesters
This course introduced students to currents aspects of plant Tissue culture technology used in
Agriculture. Plant Tissue culture is an important tool for both basic and applied aspects of plant
biotechnology as well as its commercial applications. As a technique widely known for the production of
large numbers of genetically identical plantlets, this technology exhibits several advantages over
conventional propagation techniques. It is a valuable tool for research on morphogenesis, cell signaling,
physiology, and molecular biology, as well as crop improvement by biotechnology. Plant tissue culture
who have a focus on commercial applications such as crop improvement, secondary metabolite production,
and various strategies for inducing genetic interference; persons who want to understand basic laboratory
setup, handling of explants tissue, nutrient medium and establishing the culture, and incubation of
1 Year Certificate Course in Plant Tissue culture
Duration – 1 year (2 SEM)
Eligibility – A candidate must have passed 12
from recognized Board
Fees – 2000/-Syllabus
Marks 100 =50 Theory+50 Practical (20 Assignment +30 Practical)
Unit 1
Introduction - History, Scope and Concepts of basic techniques in plant tissue culture. Laboratory
requirements and organization. Sterilization - filter, heat, wet and chemical. Media preparation – inorganic
nutrients, organic supplements, carbon source, vitamins, gelling agents, phytohormones and growth regulators;
composition of commonly used culture media (MS and Gamborg’s)
Unit 2
Types of Culture : Single cell culture, cell suspension culture, Protoplast culture, Artificial seed,
Organogenesis, Callus culture
Unit 3
Micropropagation - Factors affecting morphogenesis and proliferation rate; technical problems in
micropropagation, Production of virus free plants by meristem and shoot-tip culture
Visit to National and International Plant tissue Culture Laboratories
Hands on practical: Commercial applications of plant tissue culture and nursery technology
1. Sterilization Techniques – Autoclave and Hot Air Oven,
2. Preparation of nutrient media.
3. Establishment of callus culture.
4. Organogenesis in callus cultures
5. Test tube plants
6. Micro propagation.
7. Isolation of plant secondary metabolites.
8. Importance of macro and micro nutrients, phytohormones, growth factors in Nursery
Marks 100 =50 Theory+ 50 Job Training Project Practical)
Unit 1
Introduction to hands on techniques in plant tissue culture, preparation, handling, and establishing aseptic
Cultures; Explants selection, sterilization and inoculation; Callus and cell suspension cultures; Induction And
growth parameters.
Unit 2
Genetic manipulation of plants, genetic transformation by Agrobacterium tumefaciens. Transgenic plants for
crop improvement
Unit 3
Plant nursery technology, elite plants for propagation, condition for establishments and maintenance of
Nursery stock, Nutritional requirements of nursery stock, Macronutrients, micronutrients, Organic
Supplements, phytohormones and growth factors, mass production of nursery plants.
One month Job Training Project in National and International Plant tissue Culture Laboratories
Process for filling up of Admission Form online
  1. Read the instructions carefully before filling up the form.
  2. Accept the terms & conditions below by clicking the button "Accept & Go".
  3. To Enter %age of Marks (If Field is available in the application form) :- e.g. If %age is 78.00 then enter 78 only, If %age is 71.45 then enter 71 only and if %age is 67.67 then enter 68.
  4. Fill up the application form completely & submit it.
  5. After the form is filled up the candidate will be allotted a registration number and user ID & password from the confirmation page. Kindly note down the user ID & password to know about your candidature status.
  6. The candidate will upload her passport size photograph (less than 1 MB).
  7. After uploading the photograph you can download your Acknowledgement, Pay Pros. Fee Online & Download the Receipt..
  8. Any mistake done by the applicant is at her own risk. Admission Committee will not entertain any queries in this regard.
  9. Once the merit list is declared the candidate may check the status of through her login.
  10. The selected candidates may take admission by paying the admission fees Online from Login & Download the Receipt.
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